Single-concentration cell exposure system is an in vitro cell porous exposure system for inhalable substances based on ALI (gas-liquid interface culture exposure) technology. The system can accurately control sample dilution and exposure flow. The flow rate of each pore canal is evenly distribute; At the same time, it provides static or dynamic nutrient solution supply and constant temperature experimental environment for experimental cells. One experiment can complete multiple parallel samples of cell exposure. It has now been used by China CDC, Institute of Radiology, Military Medical Academy, Institute of Environmental Toxicology, Health and Nutrition, Military Medical Academy, Suzhou University, Beijing Cigarette Factory, Hunan Zhongyan and other units.
01.Smoke Inhalation and Health Research
02.Environmental and Health Effects of Chemicals and Pesticides
03.Influence of Air Particulate Matter and Microorganism on Environment and Health
04.Health Assessment of Air in Space
05.Study on Biosafety Evaluation of Nanomaterials
06.Impact of Industrial Chemicals on Health
07.Study on Chronic Obstructive Pulmonary Disease, Lung Cancer and Other Related Diseases
08.Related Research on Pharmaceutical Products
1. Direct exposure to monolayer cultured cells or bacteria.
2. Completely mimics the exposure of the gas-liquid level in the body environment.
3. Aerosol in the exposed chamber is uniformly distributed and flows at a constant speed.
4. Ensure consistency of flow rate and concentration of the same concentration.
5. The biosafety cabinet provides a highly clean experimental environment, and all components of the exposure module can be disassembled and disinfected.
6. Adding aerosol mass concentration detection instruments can adjust and accurately control the exposure concentration of flue gas or aerosol in real time.
7. Flow control is accurate, reaching ±1.5%.
8. The experimental efficiency is high, and a maximum of 12 parallel cells can be exposed in one experiment.
9. Unique design of constant temperature heating water tank, water bath temperature control accuracy of ±0.3℃.
10. Industrial flat panel industrial control computer, touch screen operation, stable performance, small and beautiful appearance.
11. Superior man-machine interface and highly intelligent control software, friendly and beautiful interface, simple user operation.
12. Quiet air pump is adopted to effectively reduce the noise of the instrument.
13. The module structure is reasonable and simplified, and mature unit modules are adopted to ensure the reliability and accuracy of system experiments and reduce the failure rate of the system.
14. Strong research and development technical background and a large amount of literature support.
1. Sample Generation System Module
The system provides a variety of sample generation system modules for users to choose from: intelligent smoking machine, liquid aerosol generator, dust aerosol generator, VOC gas quantitative generator, etc.
2. Sample Supply and Delivery System Module
The main function of the sample supply and delivery system module is to deliver samples with stable concentration, air pressure and flow rate for the cell exposure experiment. The delivered samples shall be clean and maintain the consistency of physical and chemical characteristics, and the uniformity of each channel in the same exposure unit shall be good to ensure the consistency of the experiment.
3. Cell Poisoning System Module
1）Clean sterile experimental environment
The cell exposure experiment is placed in a biosafety cabinet, which provides a cell exposure experiment platform and is isolated by continuously providing clean and sterile air flow.
The experimental platform is in contact with the outside world to ensure the cleanness and sterility of the experimental space, prevent external pollution and affect the experimental effect and authenticity.
2）Various cell exposure unit modules meet different test requirements.
3）Nutrient solution supply
Through the nutrient solution supply unit, the cell nutrient medium is statically or dynamically circularly supplied to the cells to ensure a good living environment for the cells.
4）Constant temperature water bath culture
According to the setting, the water bath box is provided during the experiment.
Constant temperature water ensures cells to grow in a comfortable environment.
The control accuracy of constant temperature water bath is ±0.3℃.
The interaction between the user and the instrument can be realized through the upper computer software.
4. Aerosol Mass Concentration Detection System (Optional)
Consisting of a mass concentration detector or a Shi Ying crystal microbalance (QCM) on-line mass concentration detector, aerosol particle distribution and concentration trend can be observed in real time.
CEL-712 measurement range: 0.001mg/m3to 250g/m3 (250000mg/m3); QCM measurement range: 1ng/cm2 to 300mg/cm2
5.Exhaust gas treatment system module
Special laboratory waste gas treatment system is simple to operate, convenient for centralized management and low in operation cost. Exhaust emissions fully meet national safety emission standards.
2017：The use of a 0.20 μm particulate matter filter decreases cytotoxicity in lung epithelial cells following air-liquid interfa.
2017：Genotoxicity evaluation of carbon monoxide and 1, 3-butadiene using a new joint technology — the in vitro γH2AX HCS assay combined with air-liquid interface system